Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae

نویسندگان

  • Tohru Miyoshi-Akiyama
  • Kayoko Hayakawa
  • Norio Ohmagari
  • Masahiro Shimojima
  • Teruo Kirikae
چکیده

Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78 novel STs found in the clinical isolates. These findings supported the robustness of the MLST scheme developed in this study.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Clonal distribution of multidrug-resistant Enterobacter cloacae.

A multilocus sequence typing (MLST) scheme including 7 housekeeping genes was used to evaluate whether the current spread of multidrug-resistant Enterobacter cloacae isolates worldwide might be associated to specific successful clones. Fifty E. cloacae clinical isolates of worldwide origin, with various β-lactamase content, and recovered at different periods of time were studied. Forty-four seq...

متن کامل

Multilocus Sequence Typing (MLST) of Enterococcus faecalis clinical isolates in Ilam, Iran

Introduction: The Enterococcus faecalis (E. faecalis) is the one of the pathogenic bacteria that become famous and considerable in the recent years. Here we tried to do typing the E. faecalis isolates to provide advantageous information that can help us to understand epidemiological communication between the E. faecalis isolates. Materials and methods: One hundred  E. faecalis were isolated fr...

متن کامل

Phenotypic and molecular characterization of antimicrobial resistance in Enterobacter spp. isolates from companion animals in Japan

The emergence of antimicrobial resistance among Enterobacter spp., including resistance to extended-spectrum cephalosporins (ESC), is of great concern in both human and veterinary medicine. In this study, we investigated the prevalence of antimicrobial resistance among 60 isolates of Enterobacter spp., including E. cloacae (n = 44), E. aerogenes (n = 10), and E. asburiae (n = 6), from clinical ...

متن کامل

Whole-Genome Multilocus Sequence Typing of Extended-Spectrum-Beta-Lactamase-Producing Enterobacteriaceae.

Molecular typing has become indispensable in the detection of nosocomial transmission of bacterial pathogens and the identification of sources and routes of transmission in outbreak settings, but current methods are labor-intensive, are difficult to standardize, or have limited resolution. Whole-genome multilocus sequence typing (wgMLST) has emerged as a whole-genome sequencing (WGS)-based gene...

متن کامل

تعیین توالی در چند ناحیه ژنی (MLST): یک روش تایپینگ مولکولی با قدرت تمایز بسیار بالا جهت شناسایی گونه‌های کاندیدا آلبیکنس در مطالعات اپیدمیولوژیکی

Candida spp. are ubiquitous yeasts commonly. Those are commensally and opportunist pathogens in humans. Candidiasis in immunocompromised patients and patients with severe underlying diseases or critical illnesses need aggressive diagnosis or treatment procedures. Candida albicans (C. albicans) is the most frequently isolated species. For prevention of nosocomial infections caused by C. albicans...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 8  شماره 

صفحات  -

تاریخ انتشار 2013